8EY6

LaM domain of human LARP1 in complex with AAAAAG RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.03321
Spacegroup name	P 21 21 21
Unit cell lengths	36.523, 46.187, 58.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.340 - 1.630
R-factor	0.2105
Rwork	0.209
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7soo
RMSD bond length	0.007
RMSD bond angle	0.982
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.630	1.630
Number of reflections	12601	629
<I/σ(I)>	16.6	1.5
Completeness [%]	97.2	96.3
Redundancy	6	4.8
CC(1/2)	1.000	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M Bis-Tris pH 6.5, 2 M ammonium sulfate