8EXW
Drosophila melanogaster indirect flight muscle myosin II (subfragment-1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.394, 126.806, 92.581 |
Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
Resolution | 43.480 - 2.500 |
R-factor | 0.1908 |
Rwork | 0.188 |
R-free | 0.24470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | various |
RMSD bond length | 0.003 |
RMSD bond angle | 0.524 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | BALBES |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmeas | 0.064 | |
Rpim | 0.033 | |
Number of reflections | 31441 | 1630 |
<I/σ(I)> | 11.1 | |
Completeness [%] | 99.1 | |
Redundancy | 3.7 | |
CC(1/2) | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 80% Hampton Research Index screen H11: 0.1 M Potassium thiocyanate, 30 %w/v Polyethylene glycol monomethyl ether 2,000 20% Hampton Research additive screen C3: 0.1 M Phenol |