8EW9

Crystal structure of Saccharomyces cerevisiae Altered Inheritance rate of Mitochondria protein 46 (AIM46p)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03321
Spacegroup name	P 1 21 1
Unit cell lengths	39.870, 71.350, 40.220
Unit cell angles	90.00, 95.17, 90.00

Refinement procedure

Resolution	40.060 - 2.000
R-factor	0.1736
Rwork	0.167
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7RSG
RMSD bond length	0.007
RMSD bond angle	0.773
Data reduction software	XDS (VERSION Jan 26, 2018 BUILT=20180409)
Data scaling software	XSCALE (VERSION Jan 26, 2018 BUILT=20180409)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.060	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.123	1.159
Rmeas	0.134	1.253
Rpim	0.051	0.473
Number of reflections	15174	1471
<I/σ(I)>	11.51	1.36
Completeness [%]	99.6	98.92
Redundancy	6.8	6.9
CC(1/2)	0.998	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Crystals grew from 180 nL 31.4 mg/mL Aim46p Nd62, 20 nL microseeds in 30% PEG 2000, 0.1 M MES buffer, pH 6.0, mixed with 300 nL reservoir solution composed of 18% PEG 2000, 100 mM MES buffer, pH 6.5. Microseeded crystallization plates were set with a Mosquito crystallization robot in a MRC SD2 crystallization plate. Crystals were cryopreserved with reservoir solution supplemented with 30% PEG 2000