8EVF

HUMAN DNA POLYMERASE ETA EXTENSION COMPLEX WITH AN INCOMING DCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12723
Spacegroup name	P 61
Unit cell lengths	98.671, 98.671, 81.736
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.530 - 2.870
R-factor	0.2063
Rwork	0.204
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l1i
RMSD bond length	0.004
RMSD bond angle	0.745
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.920
High resolution limit [Å]	2.870	2.870
Rmerge	0.106	0.690
Rpim	0.049
Number of reflections	10308	536
<I/σ(I)>	15.68	1.98
Completeness [%]	98.5	99.3
Redundancy	5.4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.1 M MES PH 5.5, 5mM calcium chloride, 15-22% PEG 2000 MME