8EUQ
Crystal structure of HLA-DRA*01:01/HLA-DRB1*04:01 in complex with c44H10 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 169.150, 178.720, 182.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.140 |
High resolution limit [Å] | 3.090 | 3.090 |
Rmerge | 0.159 | 0.878 |
Rpim | 0.041 | 0.226 |
Number of reflections | 50733 | 2369 |
<I/σ(I)> | 14.8 | 1.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 16.1 | 16.1 |
CC(1/2) | 0.990 | 0.709 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.7 M ammonium sulfate, 15% glycerol, 0.085 M HEPES, 1.7% PEG400, with crystal seeds previously grown in 2 M ammonium sulfate, 0.1 M bis-tris pH 5.5 |