8EQK

Human PU.1 ETS-Domain (165-270) Bound to d(AATAACCGGAAGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920105
Spacegroup name	P 1 21 1
Unit cell lengths	43.115, 60.640, 44.456
Unit cell angles	90.00, 116.17, 90.00

Refinement procedure

Resolution	23.870 - 1.450
R-factor	0.1518
Rwork	0.150
R-free	0.17940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8e3k
RMSD bond length	0.008
RMSD bond angle	1.115
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.870	1.502
High resolution limit [Å]	1.450	1.450
Rmerge	0.044	0.452
Rmeas	0.049	0.499
Rpim	0.021	0.210
Number of reflections	36361	3588
<I/σ(I)>	16.87	2.64
Completeness [%]	99.5	99.2
Redundancy	5.2	5.4
CC(1/2)	0.999	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Sodium Acetate, pH=4.6, 2% PEG 3350