8EQA

Crystal structure of human anti-N1 neuraminidase 2H08 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03321
Spacegroup name	P 2 21 21
Unit cell lengths	63.558, 110.568, 130.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.560 - 2.550
R-factor	0.2181
Rwork	0.215
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pzf 5uby
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	2.600
High resolution limit [Å]	2.550	2.550
Rpim	0.080	0.390
Number of reflections	30277	1431
<I/σ(I)>	8.6	1
Completeness [%]	98.3
Redundancy	5.9
CC(1/2)	0.983	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Zn acetate, 20% PEG3350