8EO8

Cross-reactive 3180 TCR recognition of HLA-B*35:01-NP8 epitope from 2005 H1N1 influenza strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95365
Spacegroup name	P 1 21 1
Unit cell lengths	168.088, 79.468, 172.173
Unit cell angles	90.00, 95.53, 90.00

Refinement procedure

Resolution	48.947 - 2.300
R-factor	0.182718374785
Rwork	0.181
R-free	0.21859
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lks
RMSD bond length	0.014
RMSD bond angle	1.662
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.380
High resolution limit [Å]	2.300	2.300
Rmeas	0.057	0.396
Number of reflections	201262	20019
<I/σ(I)>	13.1	2.9
Completeness [%]	100.0	100
Redundancy	2
CC(1/2)	0.998	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	PEG, ACETATE