8EN0

Structure of GII.17 norovirus in complex with Nanobody 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	I 2 2 2
Unit cell lengths	74.820, 85.459, 223.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.960 - 2.990
R-factor	0.2521
Rwork	0.249
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f4o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	111.926	111.926	3.170
High resolution limit [Å]	2.990	8.940	2.990
Rmerge	0.286	0.054	1.859
Rmeas	0.298	0.057	1.934
Total number of observations	188769
Number of reflections	14280	630	2314
<I/σ(I)>	9.1	33.92	1.31
Completeness [%]	95.5	99.5	97.9
Redundancy	13.219	12.16	13.34
CC(1/2)	0.994	0.994	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.8 M ammonium sulfate and 0.1M citric acid [pH 4]