8EMZ
Structure of GII.17 norovirus in complex with Nanobody 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.961, 90.545, 59.397 |
| Unit cell angles | 90.00, 116.29, 90.00 |
Refinement procedure
| Resolution | 48.850 - 1.400 |
| R-factor | 0.1959 |
| Rwork | 0.195 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f4o |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.530 | 4.190 |
| High resolution limit [Å] | 1.400 | 2.970 |
| Number of reflections | 85355 | 2485 |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 98.5 | |
| Redundancy | 7.009 | 7.015 |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M bicine [pH 9] and 30% [w/v] PEG6000 |
