8EMI

Crystal structure of a HLA-B*35:01-NP8 epitope from 2005 H1N1 influenza strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	50.869, 81.528, 109.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.228 - 1.573
R-factor	0.177453306722
Rwork	0.176
R-free	0.20769
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lks
RMSD bond length	0.014
RMSD bond angle	1.495
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.300	1.630
High resolution limit [Å]	1.570	1.570
Rmeas	0.042	0.394
Number of reflections	63452	6187
<I/σ(I)>	20.9	2.82
Completeness [%]	99.0
Redundancy	2
CC(1/2)	0.999	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	297	PEG