8EMD
Human PU.1 ETS-Domain (165-270) Q226E Mutant Bound to d(AATAAGCGGAAGTGGG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.009, 60.549, 44.566 |
Unit cell angles | 90.00, 116.72, 90.00 |
Refinement procedure
Resolution | 33.260 - 1.550 |
R-factor | 0.1649 |
Rwork | 0.162 |
R-free | 0.20150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8e3k |
RMSD bond length | 0.010 |
RMSD bond angle | 1.236 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.260 | 1.605 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.045 | 0.495 |
Rmeas | 0.054 | 0.591 |
Rpim | 0.029 | 0.320 |
Number of reflections | 28806 | 2825 |
<I/σ(I)> | 14.1 | 2.29 |
Completeness [%] | 96.8 | 96.35 |
Redundancy | 3.3 | 3.3 |
CC(1/2) | 0.998 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100 mM Sodium Acetate, pH=4.6, 2% PEG 3350 |