8ELZ
HRAS R97M Crystal Form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-02-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5178 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.986, 88.986, 135.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.290 - 1.961 |
| Rwork | 0.157 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k8y |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.990 |
| High resolution limit [Å] | 1.960 | 5.320 | 1.960 |
| Rmerge | 0.111 | 0.040 | 0.796 |
| Rmeas | 0.117 | 0.042 | 0.871 |
| Rpim | 0.036 | 0.013 | 0.340 |
| Total number of observations | 149776 | ||
| Number of reflections | 14971 | 821 | 654 |
| <I/σ(I)> | 5.3 | ||
| Completeness [%] | 99.3 | 100 | 90.1 |
| Redundancy | 10 | 10.2 | 5.6 |
| CC(1/2) | 0.999 | 0.656 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, Calcium Acetate, Magnesium Chloride, Sodium Chloride, DTT |
