8ELW
HRAS R97A Crystal Form 1 T-State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.226, 88.226, 133.839 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.650 - 1.699 |
| R-factor | 0.1628 |
| Rwork | 0.160 |
| R-free | 0.18630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k8y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.699 | 3.660 | 1.700 |
| Rmerge | 0.060 | 0.034 | 0.229 |
| Number of reflections | 22327 | 2338 | 2204 |
| <I/σ(I)> | 14.2 | ||
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 10.9 | 10.5 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, Calcium Acetate, Sodium Chloride, Magnesium Chloride, HEPES |
