8ELR

HRAS R97F Crystal Form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-09-16
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 32 2 1
Unit cell lengths	39.258, 39.258, 157.984
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.000 - 2.050
Rwork	0.188
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctq
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.18.2)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.090
High resolution limit [Å]	2.050	5.560	2.050
Rmerge	0.051	0.029	0.291
Rmeas	0.054	0.031	0.315
Rpim	0.018	0.011	0.119
Total number of observations	86621
Number of reflections	9528	568	469
<I/σ(I)>	11.3
Completeness [%]	99.3	99.6	100
Redundancy	9.1	8.3	6.8
CC(1/2)		1.000	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Calcium Acetate PEG3350, Magnesium Chloride, HEPES, Sodium Chloride, DTT