8EL4
Light harvesting phycobiliprotein HaPE555 from the cryptophyte Hemiselmis andersenii CCMP644 in a tight interface filament
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.529, 71.258, 48.283 |
Unit cell angles | 90.00, 108.47, 90.00 |
Refinement procedure
Resolution | 45.800 - 1.730 |
R-factor | 0.1902 |
Rwork | 0.188 |
R-free | 0.23920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lmx |
RMSD bond length | 0.013 |
RMSD bond angle | 1.415 |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHENIX (1.14) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.800 | 1.760 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.135 | |
Number of reflections | 42700 | 2325 |
<I/σ(I)> | 8.2 | 0.7 |
Completeness [%] | 100.0 | |
Redundancy | 6.5 | |
CC(1/2) | 0.997 | 0.355 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 300 | PEG3350 25% (w/v) + 0.01M Sarcosine, 150 nL mother + 150 nL protein solution, Cryoprotectant of 20% Gylcerol |