8EI8
Crystal structure of the WWP2 HECT domain in complex with H308, a Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 111.380, 117.810, 65.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.770 - 2.900 |
| R-factor | 0.2399 |
| Rwork | 0.237 |
| R-free | 0.30670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y07 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.770 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.248 | |
| Number of reflections | 9862 | 1564 |
| <I/σ(I)> | 8 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.2 | 13.8 |
| CC(1/2) | 0.992 | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2M Magnesium Chloride, 0.1M Bis-Tris pH 6.5, 20% PEG3350 |
