8EI2
Crystal structure of the N-terminal domain of CUL5 in complex with H314, a Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 6 |
| Unit cell lengths | 173.030, 173.030, 57.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.880 - 2.800 |
| R-factor | 0.3398 |
| Rwork | 0.339 |
| R-free | 0.35750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wzk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.880 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.322 | |
| Number of reflections | 24154 | 3518 |
| <I/σ(I)> | 5 | 1.8 |
| Completeness [%] | 98.2 | 99.3 |
| Redundancy | 13.2 | 13.7 |
| CC(1/2) | 0.988 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 30% w/v PEG 8000, 0.1M MES Sodium Salt pH6.5, 0.2M Ammonium Sulfate, 4% v/v 1,3-Propanediol |
