8EG3

Structure of human placental steroid (estrone/DHEA) sulfatase at 2.0 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-28
Detector	ADSC QUANTUM 210
Wavelength(s)	0.98
Spacegroup name	P 3 2 1
Unit cell lengths	117.190, 117.190, 102.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.130 - 2.040
R-factor	0.2211
Rwork	0.220
R-free	0.25050
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1p49
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.180	2.150
High resolution limit [Å]	2.040	2.040
Rmerge	0.081	0.690
Number of reflections	51319	7486
<I/σ(I)>	13.1	2.8
Completeness [%]	99.0	100
Redundancy	6.5	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	MPD, ammonium phosphate, BOG, Tris buffer