8EFX
Structure of OtDUB DUB Domain disulfide crosslinked with Ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 42.399, 83.008, 99.354 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.700 - 1.850 |
R-factor | 0.1998 |
Rwork | 0.198 |
R-free | 0.22920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq 6ups |
RMSD bond length | 0.007 |
RMSD bond angle | 0.792 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.700 | 1.920 |
High resolution limit [Å] | 1.840 | 1.850 |
Number of reflections | 30867 | 3002 |
<I/σ(I)> | 11.57 | |
Completeness [%] | 99.7 | |
Redundancy | 6.56 | |
CC(1/2) | 0.997 | 0.481 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.16M Magnesium chloride, 0.08M Tris at pH 8.5, 24% PEG 8000 and 20% glycerol |