8EEZ

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ100-C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	55.981, 71.226, 114.176
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.896 - 2.250
R-factor	0.1879
Rwork	0.185
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fqq
RMSD bond length	0.009
RMSD bond angle	1.196
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.190	2.190
Number of reflections	22274	1968
<I/σ(I)>	7.6
Completeness [%]	92.5
Redundancy	4.1
CC(1/2)	0.990	0.579

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	22.5% PEG 4K, 22.5% IPA, 0.1M Na Citrate (pH5.6)