8ECE
E. coli L-asparaginase II mutant (V27T) in complex with L-Glu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.772, 126.954, 132.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.440 - 1.860 |
| R-factor | 0.1436 |
| Rwork | 0.143 |
| R-free | 0.19150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eca |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.008 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.107 | 0.791 |
| Rmeas | 0.111 | 0.839 |
| Rpim | 0.030 | 0.271 |
| Number of reflections | 105359 | 5199 |
| <I/σ(I)> | 26.15 | 2.08 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 12.8 | 8.3 |
| CC(1/2) | 0.998 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.14 M Sodium bromide, 14% w/v Polyethylene glycol 3,350 |
