8ECA

Covalently stabilized triangular trimer derived from Abeta16-36

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	113
Detector technology	CCD
Collection date	2016-07-16
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	43.537, 43.537, 93.329
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.730 - 2.270
R-factor	0.2699
Rwork	0.265
R-free	0.31810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ec9
RMSD bond length	0.006
RMSD bond angle	1.127
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.730	2.355
High resolution limit [Å]	2.270	2.270
Number of reflections	8927	489
<I/σ(I)>	28.66
Completeness [%]	98.4
Redundancy	41.3
CC(1/2)	1.000	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296.15	0.1 M Tris at pH 8.3, 0.2 M ammonium acetate, and 39% 2-methyl-2,4-pentanediol (MPD)