8EB5

Tandem of Hermes transposase BED domain in complex with the quasi palindrome of its transposon left-end

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-07-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.28335
Spacegroup name	P 65 2 2
Unit cell lengths	55.271, 55.271, 212.573
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.640 - 2.500
R-factor	0.2316
Rwork	0.231
R-free	0.23820
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	BUSTER (2.10.4 (11-DEC-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.640	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.044	1.224
Rmeas	0.046	1.224
Rpim	0.011	0.272
Number of reflections	7292	694
<I/σ(I)>	35.47	2.39
Completeness [%]	99.8	100
Redundancy	19.2	19.23
CC(1/2)	0.999	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG 4000, Bis-TRIS