8EB2
Structure of HLA-A*02:01 in complex with NY-ESO-1 peptide and PA2.1 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 116.800, 148.790, 199.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.600 | 3.010 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 145809 | 7620 |
<I/σ(I)> | 7.19 | 0.9 |
Completeness [%] | 99.1 | 95.2 |
Redundancy | 6.7 | |
CC(1/2) | 0.990 | 0.381 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M L-Proline, 0.1M HEPES pH 7.5, 13% PEG3350 |