8E8V
Structure of the short LOR domain of human AASS bound to N-ethylsuccinimide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-16 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.92010 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.099, 155.963, 78.352 |
Unit cell angles | 90.00, 100.20, 90.00 |
Refinement procedure
Resolution | 47.490 - 2.450 |
R-factor | 0.2351 |
Rwork | 0.234 |
R-free | 0.25780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8e8t |
Data reduction software | autoPROC |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.490 | 47.490 | 2.510 |
High resolution limit [Å] | 2.450 | 11.490 | 2.450 |
Rmerge | 0.148 | 0.069 | 2.378 |
Rmeas | 0.164 | 0.077 | 2.644 |
Rpim | 0.071 | 0.033 | 1.144 |
Total number of observations | 350595 | 3412 | 23252 |
Number of reflections | 66842 | 670 | 4452 |
<I/σ(I)> | 6.5 | 19.1 | 0.8 |
Completeness [%] | 99.9 | 98.7 | 99.2 |
Redundancy | 5.2 | 5.1 | 5.2 |
CC(1/2) | 0.994 | 0.992 | 0.428 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | 0.2 M NaCl, 0.1M HEPES, 25% PEG 4000 |