8E8P
GSTZ1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.541780 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.056, 50.079, 54.645 |
| Unit cell angles | 83.47, 69.76, 73.68 |
Refinement procedure
| Resolution | 26.682 - 2.281 |
| R-factor | 0.2544 |
| Rwork | 0.252 |
| R-free | 0.29560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fw1 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.280 | 6.240 | 2.300 |
| Rmerge | 0.071 | 0.037 | 0.492 |
| Rmeas | 0.087 | 0.045 | 0.611 |
| Rpim | 0.050 | 0.026 | 0.358 |
| Number of reflections | 17447 | 891 | 865 |
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 96.3 | 98.9 | 94.7 |
| Redundancy | 3 | 2.8 | 3 |
| CC(1/2) | 0.996 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MgCl2; 0.1M alpha-D-galacturonic acid, pH 6.5; 12% w/v PEG6000 |
