8E8B
Human DNA polymerase eta-DNA-rU-ended primer ternary mismatch complex:ground state at pH7.0 (K+ MES) with 1 Ca2+ ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97618 |
Spacegroup name | P 61 |
Unit cell lengths | 97.870, 97.870, 82.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.040 - 2.200 |
R-factor | 0.1976 |
Rwork | 0.195 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7m7l |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.040 | 32.040 | 2.070 |
High resolution limit [Å] | 2.020 | 9.030 | 2.020 |
Rmerge | 0.081 | 0.049 | 0.814 |
Rmeas | 0.084 | 0.050 | 0.908 |
Total number of observations | 529123 | ||
Number of reflections | 50583 | 634 | 1241 |
<I/σ(I)> | 17.13 | 50.4 | 1.7 |
Completeness [%] | 87.5 | 99.4 | 28.9 |
Redundancy | 10.46 | 15.473 | 4.618 |
CC(1/2) | 0.999 | 0.999 | 0.648 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 2000MME, 0.1 M MES |