8E8A

Human DNA polymerase eta-DNA-dT-ended primer binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.127130
Spacegroup name	P 61
Unit cell lengths	98.291, 98.291, 82.076
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.950 - 1.800
R-factor	0.2067
Rwork	0.206
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.950	29.950	1.910
High resolution limit [Å]	1.800	5.380	1.800
Rmerge	0.098	0.072	0.870
Rmeas	0.103	0.076	0.919
Total number of observations	837629
Number of reflections	81616	3080	13102
<I/σ(I)>	13	31.31	2.23
Completeness [%]	99.9	99.9	99.5
Redundancy	10.263	11.05	9.565
CC(1/2)	0.998	0.996	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES