8E8A
Human DNA polymerase eta-DNA-dT-ended primer binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.127130 |
Spacegroup name | P 61 |
Unit cell lengths | 98.291, 98.291, 82.076 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.950 - 1.800 |
R-factor | 0.2067 |
Rwork | 0.206 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7m7l |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.950 | 29.950 | 1.910 |
High resolution limit [Å] | 1.800 | 5.380 | 1.800 |
Rmerge | 0.098 | 0.072 | 0.870 |
Rmeas | 0.103 | 0.076 | 0.919 |
Total number of observations | 837629 | ||
Number of reflections | 81616 | 3080 | 13102 |
<I/σ(I)> | 13 | 31.31 | 2.23 |
Completeness [%] | 99.9 | 99.9 | 99.5 |
Redundancy | 10.263 | 11.05 | 9.565 |
CC(1/2) | 0.998 | 0.996 | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 2000MME, 0.1 M MES |