8E86

Human DNA polymerase eta-DNA-rC-ended primer-dGMPNPP ternary mismatch complex with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.127230
Spacegroup name	P 61
Unit cell lengths	98.380, 98.380, 82.063
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.200 - 1.780
R-factor	0.1949
Rwork	0.194
R-free	0.20450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.200	32.200	1.890
High resolution limit [Å]	1.780	5.320	1.780
Rmerge	0.133	0.060	1.260
Rmeas	0.140	0.063	1.322
Total number of observations	893092
Number of reflections	84523	3178	13573
<I/σ(I)>	14.02	42.2	2.1
Completeness [%]	99.9	99.8	99.5
Redundancy	10.566	10.938	10.736
CC(1/2)	0.998	0.998	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES