8E84
Human PCNA mutant- C148S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-01 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.641, 80.871, 70.047 |
| Unit cell angles | 90.00, 117.52, 90.00 |
Refinement procedure
| Resolution | 34.970 - 3.100 |
| R-factor | 0.257 |
| Rwork | 0.255 |
| R-free | 0.29210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e0t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.764 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.010 |
| High resolution limit [Å] | 2.960 | 8.020 | 2.960 |
| Rmerge | 0.141 | 0.047 | 0.718 |
| Total number of observations | 32581 | ||
| Number of reflections | 12889 | 724 | 371 |
| <I/σ(I)> | 5.4 | ||
| Completeness [%] | 90.3 | 97.4 | 53.5 |
| Redundancy | 2.5 | 2.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 175 mM Magnesium Acetate, 20% PEG 3350 |
