8E7W
RsTSPO A139T with Heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.399, 99.568, 95.812 |
| Unit cell angles | 90.00, 100.16, 90.00 |
Refinement procedure
| Resolution | 28.950 - 2.100 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uc1 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.950 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.078 | 0.482 |
| Number of reflections | 31427 | 3098 |
| <I/σ(I)> | 6 | 2 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 2 | |
| CC(1/2) | 0.992 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 28-30% PEG400, HEPES (pH 8.5), 100mM sodium acetate, 100 mM ammonium acetate |
