8E2W

Structure of CRISPR-Associated DinG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.88684
Spacegroup name	P 65
Unit cell lengths	123.311, 123.311, 136.874
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.740 - 2.950
R-factor	0.1873
Rwork	0.185
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold Model
RMSD bond length	0.002
RMSD bond angle	0.518
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.060
High resolution limit [Å]	2.950	2.950
Rmerge		1.356
Rpim		0.346
Number of reflections	24916	2453
<I/σ(I)>	20	2
Completeness [%]	100.0	99.8
Redundancy	20.5	16
CC(1/2)	0.997	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	225 mM Imidazole pH 8.0, 3.5% PEG8000, and a 30% sucrose mixture. The crystal used for structure determination comprised 1 uL (5mg/ml) protein solution to 2.6 uL of mother liquor and 0.4 uL sucrose