8E13
Structures of HLA-B8E76C loaded with long peptides reveal novel features at the N-terminus of the groove
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2020-08-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.577, 81.273, 110.498 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.000 - 1.370 |
| R-factor | 0.1682 |
| Rwork | 0.166 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6p2s |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.115 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.470 | 1.420 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.068 | 0.480 |
| Number of reflections | 95762 | 4263 |
| <I/σ(I)> | 29.1 | |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 4.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 0.2 M ammonium acetate, 18% PEG 4000, 0.1 M sodium citrate, |
