8E10
Structure of mouse polymerase beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS 3.0 MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-27 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.725, 120.534, 69.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.520 - 1.650 |
| R-factor | 0.1775 |
| Rwork | 0.173 |
| R-free | 0.21420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zqy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.052 |
| Data reduction software | SAINT (V8.40B) |
| Data scaling software | Aimless (0.7.8) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.726 | 1.680 |
| High resolution limit [Å] | 1.647 | 1.647 |
| Rmerge | 0.082 | 1.031 |
| Rmeas | 0.088 | 1.126 |
| Rpim | 0.031 | 0.447 |
| Number of reflections | 63779 | 3092 |
| <I/σ(I)> | 20.4 | 1.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 8 | 6.3 |
| CC(1/2) | 0.999 | 0.625 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287.15 | 0.1 M HEPES pH 7.5, 22.5% w/v PEG3350, 1.5% v/v Tacsimate pH 7.0 |
