8DZN
Crystal structure of human Sar1a in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.172, 63.970, 62.233 |
| Unit cell angles | 90.00, 105.95, 90.00 |
Refinement procedure
| Resolution | 28.208 - 2.107 |
| R-factor | 0.1852 |
| Rwork | 0.181 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8dzm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.022 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.104 | 0.236 |
| Rmeas | 0.136 | 0.324 |
| Rpim | 0.066 | 0.159 |
| Number of reflections | 24060 | 1138 |
| <I/σ(I)> | 17.57 | 6.2 |
| Completeness [%] | 95.8 | 88.7 |
| Redundancy | 4.1 | 3.9 |
| CC(1/2) | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.5M NaH2PO4/K2HPO4 and 0.1M sodium citrate, pH5.5 |
