8DZK

Dbr1 in complex with 5-mer cleavage product

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	73.272, 143.341, 214.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.120 - 2.100
R-factor	0.1794
Rwork	0.178
R-free	0.20920
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5k73
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.120	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.024	0.368
Rmeas	0.034	0.520
Number of reflections	130333	12870
<I/σ(I)>	20.91	2.11
Completeness [%]	99.0
Redundancy	1.9
CC(1/2)	0.998	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	15% PEG3350, 0.4 M LiSO4, 0.1 M bis-tris pH 5.5