8DYI
IL17A homodimer bound to Compound 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.938, 30.507, 50.239 |
| Unit cell angles | 90.00, 102.71, 90.00 |
Refinement procedure
| Resolution | 38.830 - 2.280 |
| R-factor | 0.274 |
| Rwork | 0.272 |
| R-free | 0.31140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hr9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.158 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.170 | 2.272 |
| High resolution limit [Å] | 2.260 | 2.264 |
| Number of reflections | 12501 | 102 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.990 | 0.802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 290.15 | 3.2M NaCl, 0.1M Tris, pH 8.5 |
