8DYF
IL17A homodimer bound to Compound 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.260, 96.260, 51.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.340 - 2.020 |
| R-factor | 0.2161 |
| Rwork | 0.214 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hr9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.129 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.400 | 2.056 |
| High resolution limit [Å] | 2.000 | 2.022 |
| Number of reflections | 18098 | 914 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10 | |
| CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 290.15 | 3.2M NaCl, 0.1M Tris, pH 8.5 |
