8DY0

Crystal Structure of spFv GLK1 HL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	37.940, 50.740, 63.200
Unit cell angles	76.35, 89.20, 69.16

Refinement procedure

Resolution	35.350 - 2.100
R-factor	0.1842
Rwork	0.182
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3na9
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.350	35.350	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.152	0.100	0.607
Rmeas	0.167	0.109	0.721
Total number of observations	132954
Number of reflections	24805	279	1840
<I/σ(I)>	7.38	16.89	2.33
Completeness [%]	99.5	99.6	99.2
Redundancy	5.36	6.082	3.386
CC(1/2)	0.991	0.990	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	100 mM MES, 750 mM (NH4)2SO4, 5% PEG 400