8DVN

Crystal structure of LRP6 E3E4 in complex with disulfide constrained peptide E3.10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000030
Spacegroup name	P 21 21 21
Unit cell lengths	68.097, 116.783, 127.164
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.330 - 2.530
R-factor	0.2112
Rwork	0.209
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a0p
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20rc3-4406_final)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.330	2.610
High resolution limit [Å]	2.530	2.530
Rpim	0.039	0.810
Number of reflections	34563	22437
<I/σ(I)>	15.13
Completeness [%]	99.9	99.88
Redundancy	6.7	6.6
CC(1/2)	0.999	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350, 0.2 M KSCN