8DTN

The complex of nanobody 6101 with BCL11A ZF6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 31 2 1
Unit cell lengths	54.933, 54.933, 408.619
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.279 - 2.199
R-factor	0.1969
Rwork	0.195
R-free	0.23240
Structure solution method	SAD
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	BIOMOL
Phasing software	PHENIX (1.13-2998-000)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.280	2.278
High resolution limit [Å]	2.199	2.199
Rmerge	0.055	0.128
Rmeas	0.078	0.181
Rpim	0.055	0.128
Number of reflections	37430	37119
<I/σ(I)>	17.13	5.62
Completeness [%]	97.5	93.61
Redundancy	7.3	7.7
CC(1/2)	0.993	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281.15	0.1 M Bis-Tris propane, pH 6.0-7.0, 0.2 M NaKPO4 and 18%-20% PEG 3350