8DT6

Crystal Structure of DNA Polymerase III beta subunit from Elizabethkingia anophelis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-09-16
Detector	RAYONIX MX-300
Wavelength(s)	0.9787
Spacegroup name	P 1 21 1
Unit cell lengths	92.410, 88.010, 100.240
Unit cell angles	90.00, 95.75, 90.00

Refinement procedure

Resolution	40.260 - 2.350
R-factor	0.1773
Rwork	0.176
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.260	40.260	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.052	0.022	0.548
Rmeas	0.059	0.025	0.626
Total number of observations	280947
Number of reflections	66682	768	4922
<I/σ(I)>	18.5	48.97	2.73
Completeness [%]	99.8	96.2	99.8
Redundancy	4.213	3.763	4.236
CC(1/2)	0.999	0.999	0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	287	[Target: ElanA.17987.a.AE1.PS38659 - 28.82 mg/mL] [Barcode: 321211c7] [Pin: mjs7-5] [Crystallization: sparse screen - MCSG1 C7 - 100 mM Tris:HCl pH 8.5, 200 mM Calcium chloride, 25% (w/v) PEG 4000, 14C, 0.2:0.2 drop ratio] [Cryo: 15% EG]