8DSO
Structure of cIAP1, BTK and BCCov
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 104.360, 104.360, 110.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 110.290 - 2.334 |
| R-factor | 0.2446 |
| Rwork | 0.242 |
| R-free | 0.29180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p9j |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.290 | 2.734 |
| High resolution limit [Å] | 2.334 | 2.337 |
| Rpim | 0.113 | 0.551 |
| Number of reflections | 17804 | 890 |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 88.9 | |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 0.1 M BTP pH 9, 4M potassium formate, and 2% PEG MME 2K |
