8DS7
Tumor-activated antibody derivatives targeting CTLA4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-10-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 37.010, 88.490, 128.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 1.880 |
| R-factor | 0.1581 |
| Rwork | 0.156 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ioi |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.699 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmeas | 0.073 | 0.350 |
| Number of reflections | 188519 | 1 |
| <I/σ(I)> | 20.83 | |
| Completeness [%] | 98.5 | 91 |
| Redundancy | 5.4 | |
| CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Sodium Formate, 23% (w/v) PEG 3350 |
