8DQO
Crystal structure of Arabidopsis thaliana COSY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-26 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.411, 57.903, 270.395 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.760 - 1.900 |
| R-factor | 0.1916 |
| Rwork | 0.190 |
| R-free | 0.23017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g0b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.326 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.720 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 71357 | 6352 |
| <I/σ(I)> | 13.42 | |
| Completeness [%] | 98.7 | 89.64 |
| Redundancy | 1.1 | 1.1 |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 50 mM calcium acetate, 0.1 M sodium cacodylate pH 6.5, 20% glycerol |
