8DQ6

Structure of A. thaliana MIF/D-DT-like protein-1 (MDL1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9999
Spacegroup name	P 65
Unit cell lengths	66.737, 66.737, 132.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.530 - 1.560
R-factor	0.1833
Rwork	0.182
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kqx
RMSD bond length	0.006
RMSD bond angle	0.824
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.18_3845: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.530	1.620
High resolution limit [Å]	1.560	1.560
Rmerge	0.052	0.990
Rmeas	0.054
Rpim	0.012	0.236
Number of reflections	46283	4257
<I/σ(I)>	31.45
Completeness [%]	97.5	90.4
Redundancy	20
CC(1/2)	1.000	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50 mM Tris, pH 8.0, 0.2 M calcium acetate, 26% PEG8000, 30% ethylene glycol