8DPE

Crystal structure of ATP-dependent RNA helicase DDX42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-15
Detector	RAYONIX MX-225
Wavelength(s)	0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	42.917, 73.268, 134.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.147 - 1.531
R-factor	0.2142
Rwork	0.212
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nho
RMSD bond length	0.007
RMSD bond angle	1.188
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.530	3.300	1.530
Rmerge	0.056	0.029	0.838
Rmeas	0.062	0.032	0.926
Rpim	0.025	0.013	0.386
Total number of observations	384934
Number of reflections	63581	6809	6124
<I/σ(I)>	8.7
Completeness [%]	98.4	99.7	96.2
Redundancy	6.1	5.9	5.4
CC(1/2)		0.998	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	1 uL 8 mg/mL DDX42 (in 25 mM Tris, pH 7.5, 300 mM sodium chloride, 1 mM TCEP) + 1 uL reservoir solution (6-10% PEG8000, 0.1 M Tris, pH 8.5-9.0)