8DPD

superfolder GFP Tyr74pCNPhe mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	47.222, 47.222, 346.153
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.900 - 1.510
R-factor	0.2103
Rwork	0.208
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.015
RMSD bond angle	1.527
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	1.550
High resolution limit [Å]	1.510	1.510
Number of reflections	37289	1762
<I/σ(I)>	14.6	7.7
Completeness [%]	99.2
Redundancy	8.3
CC(1/2)	0.999	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Magnesium chloride, 0.1 M Tris-HCl pH 8.5, 25% PEG 3350