8DMT

Crystal structure of macrodomain CG2909 from Drosophila melanogaster in complex with ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	48.949, 90.006, 212.267
Unit cell angles	90.00, 95.89, 90.00

Refinement procedure

Resolution	38.490 - 2.280
R-factor	0.1967
Rwork	0.196
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold RE54994p
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.490	2.362
High resolution limit [Å]	2.280	2.280
Number of reflections	77326	5609
<I/σ(I)>	8.9
Completeness [%]	92.2
Redundancy	6.8
CC(1/2)	0.984	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M ammonium citrate, 12% w/v PEG3350